The cryopreserved aortal grafts in recent experiment were transplanted with the same anesthesiological and surgical techniques used previously in our cold-stored aortal grafts experiment

The cryopreserved aortal grafts in recent experiment were transplanted with the same anesthesiological and surgical techniques used previously in our cold-stored aortal grafts experiment. clinical cold-storage protocol. == Results == Cryopreserved allografts showed regular morphology of aortal wall with clear differentiation of all three basic anatomical layers on day 30 postransplant. Intimal layer showed no hyperplasia, luminal surface was covered by endothelial cells. No statistical difference was observed PHA 408 in tunica media thickness between isografts and allografts. The medial layer showed no necrosis, shrinkage or immunoglobuline G deposition in any experimental group. The adventitial infiltration by immune cells was significantly higher (P<0.05) in allografts. Cryopreserved allografts showed significant lower activation of both cell- and antibody Mouse monoclonal to PTH1R mediated immunity compared to historical data of cold-stored allografts. == Conclusion == Aortal wall histology of rat allografts treated by our new standardized clinical cryopreservation/slow thawing protocol was comparable to that of the cryopreserved isografts on day 30 posttranspant. The immunogenicity of cryopreserved aortal allografts was significantly lower compared to that of cold-stored aortal allografts. == Introduction == Incidence rates of aortic grafts contamination in patients after primary aortic surgery in the current endovascular era is usually considerable [1]. Major aortic graft contamination is usually a life-threatening complication with high mortality and morbidity rates [2,3]. One of the most effective treatment modality of this devastating complication is the reoperation with replacement of an infected prosthesis by a cold-stored [4] or cryopreserved arterial allograft [5]. The recovery techniques of arterial allografts and subsequent cold-stored or cryopreservation/thawing protocols published by large vascular centers are very inhomogeneous [6,7]. The different properties of storage solutions, variation in cold ischemic time prior to implantation (cold-stored allografts) or cryopreservation, different freezing and thawing protocols are used worldwide [46]. All these aspects can influence the final PHA 408 quality of implanted arterial allografts and caused significant differences in the early and late graft-related complications [6]. In the Czech Republic, the cold stored arterial allografts are used as a substitute for infected vascular prosthesis since middle 1990s [7]. Both solid organ and cold-stored arterial transplantation programs are governed by the same legislation and managed by the Transplantation Coordination PHA 408 Centre of the Czech Republic. PHA 408 Therefore, the cold-stored arteries obtained during multi-organ recovery from braindead donors are stored in organ conservation solutions and must be transplanted to the patient included in the special waiting list within 48 hours after recovery [8]. However, cold-stored allografts were not in sufficient abundance for PHA 408 all the patients around the waiting list. Therefore, the clinical cryopreserved arterial transplantation program was started in 2011. All of arterial allografts included to the national program are processed exclusively in the Tissue Bank of The University Hospital Hradec Kralove under new standardized cryopreservation/slow thawing protocol [9]. At the present time there are two waiting list for patients indicated for arterial transplantation in the Czech Republic. The inclusion of each patient for cold-stored or cryopreserved arterial waiting list is the preference of indicating surgeon [9]. The experimental and clinical studies revealed strong antigenicity of both cold-stored and cryopreserved allografts, respectively [10,11]. This immunogenicity is responsible for late graft-related complication presented as graft dilatation, rupture or thrombosis [12]. In addition, this immunogenicity is usually influenced by all procedure actions in their pretransplant history [10]. Therefore the aim of our study was: to transfer all actions of the new national standardized clinical cryopreservation/slow thawing protocol to the experimental settings, to study acute immune reaction of.