Secreted IL-1 was recognized in cell-free supernatants from PBMC or monocytes (enriched by adherence to plastic for 2h followed by washes to remove non-adherent cells) stimulated for 4h or 24h with 20 ng/ml or 1 g/ml of LPS; the human
In some tests, migration of uPAR-overexpressing MCF-7 cells was quantitated by repairing the membranes in methanol and staining the migratory cells with 0
In some tests, migration of uPAR-overexpressing MCF-7 cells was quantitated by repairing the membranes in methanol and staining the migratory cells with 0.1% crystal violet. least 6 h. The uPA-induced upsurge in MCF-7 cell migration was noticed selectively on vitronectin-coated