TCRGA gene rearrangements were discovered

TCRGA gene rearrangements were discovered. which is usually characterized by a complete obliteration of the lymphatic follicular structure. Fifty-two cases (95%) had tumor cells that were positive for CD3, 50 cases (91%) were positive for CD4, 33 cases (60%) were positive for Bcl-6, 20 cases (36%) were positive for CD10, 44 cases (80%) were positive for CXCL13 to different degrees, and 53 cases (96%) showed a strong positive expression of CD21. Ki67 expression intensity was 30-80% in tumor T cells. Clonal gene rearrangements were identified in 48 of the 55 angioimmunoblastic T-cell lymphoma cases (87%), of which 30 (55%) displayed IG gene rearrangements, including IGHA (7 cases; 13%), IGHB (6 cases; 11%), IGHC (2 cases; 4%), IGKA (22 cases; 40%), IGKB (6 cases; 11%), and IGL (20 cases; 36%). TCR gene AMG-1694 rearrangements AMG-1694 were observed in 32 cases (58%), including TCRBA (6 cases; 11%), TCRBB (5 cases; 9%), TCRBC (10 cases; 18%), TCRD (7 cases; 13%), TCRGA (22 cases; 40%), and TCRGB (16 cases; 29%). IG and TCR gene rearrangements were concurrently observed in 14 cases (25%). Immunoglobulin or TCR clonal gene rearrangements were not detected in the 15 cases of reactive hyperplasia. Angioimmunoblastic T-cell lymphomas may be positive for immunoglobulin or T-cell receptor clone gene rearrangements or may express double rearrangements. The assessment of clonal gene rearrangements is usually useful for the diagnosis and differential diagnosis of angioimmunoblastic T-cell lymphoma. strong class=”kwd-title” Keywords: Immunoglobulin, T cell receptor, gene rearrangements, angioimmunoblastic T-cell lymphoma Introduction Angioimmunoblastic T-cell lymphoma (AITL) is usually a rare and aggressive subtype of lymphoma but accounts for a major subset of peripheral T-cell lymphomas. AITL is usually characterized clinically by the sudden onset of its constitutional symptoms, which include lymphadenopathy, hepatosplenomegaly, hypergammaglobulinemia, and in particular, hemolytic anemia [1-5]. AITL causes a unique stromal reaction, and its pathologic characteristics include polymorphic T-cell infiltration, high venous endothelial proliferation, follicular dendritic-cell proliferation, polyclonal B-cell infiltration, and inflammatory cell infiltration. AITL is usually often associated with Epstein-Barr computer virus (EBV) contamination. The tumor AMG-1694 cells originate from the auxiliary T lymphocytes in the germinal center. The typical histologic features of AITL include the following: (1) The lymph nodes contain polymorphic small- to medium-sized lymphocytes with a transparent cytoplasm, round or ovoid nucleus, and small nucleoli. (2) There are large, scattered immunoblast-like cells, which have one or two nucleoli and a basophilic cytoplasm; (3) Rabbit polyclonal to ABHD12B There is obvious hyperplasia of the high endothelial branched vein, and the endothelial cells are swollen. The blood vessel cells with a hyaline cytoplasm are often surrounded by atypical lymphocytes. (4) There is a proliferation of follicular dendritic cells with a branch or windblown shape. (5) The background of inflammatory cells includes small lymphocytes, eosinophils, plasma cells, and histocytes. (6) The peripheral sinus of the lymph nodes is usually often present, and the peripheral fatty tissue is usually infiltrated by the tumor tissue. AITL can be categorized into three types. Type 1 is usually rare, and its early pathologic changes consist of an intact lymphoid follicle structure and T area growth only. Type 2 is usually characterized by an intact segmental lymphatic follicular structure. Type 3 is usually characterized by a complete obliteration of the lymphatic follicular structure [6]. The first two types are frequently observed in T-area reactive hyperplasia. The third type is usually relatively common and often needs to be distinguished from peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS), classical Hodgkins lymphoma, and T-cell-rich large B cell lymphoma. We used the BIOMED-2 primer design system, the IdentiClone gene rearrangement detection kit, and the GeneScanning method to assess clonality and detect immunoglobulin (IG) and T-cell receptor (TCR) gene rearrangements in 55 cases of AITL and 15 cases of reactive hyperplasia. Materials and methods Case information A total of 55 excised AITL samples collected by the pathology department at Xiangya Hospital from October 2012 to October 2016.